podj l (Viollier AG)

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Viollier AG podj l
Podj L, supplied by Viollier AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/podj+l/podj+l/pmc04002673-452-0-17
Average 90 stars, based on 1 article reviews

podj l - by Bioz Stars, 2026-09

90/100 stars

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Chemotaxis Assay:

Article Title: Cytokinesis signals truncation of the PodJ polarity factor by a cell cycle-regulated protease
Article Snippet: .. Thus, PerP appears to participate specifically in the proteolytic processing of PodJ L . The podJ gene has been shown to be required for pili biogenesis, holdfast formation, and efficient chemotaxis ( Wang et al , 1993 ; Viollier et al , 2002a ; Hinz et al , 2003 ), with PodJ L specific for pili formation and PodJ S for holdfast and chemotaxis functions ( Viollier et al , 2002a ). ..

other:

Article Title: Complex regulatory pathways coordinate cell cycle progression and development in Caulobacter crescentus
Article Snippet: PodJ L binds to the future site of pilus biogenesis and serves as a localization factor for the pilus assembly protein, CpaE ( Viollier et al., 2002b ).

Expressing:

Article Title: Cytokinesis signals truncation of the PodJ polarity factor by a cell cycle-regulated protease
Article Snippet: .. First, previous studies suggested that removal of 20–30 kDa from PodJ L 's C-terminus yields PodJ S ( Viollier et al , 2002a ; Chen et al , 2005 ), and the size of the removed fragment is consistent with that of PodJ PERI -C. Second, we examined Escherichia coli cells expressing a PodJ L construct containing GFP at its N-terminus by immunoblotting ( Supplementary Figure S6 ). ..

Construct:

Western Blot:

Sequencing:

Article Title: How do bacteria localize proteins to the cell pole?
Article Snippet: .. Conversion of PodJ from a long (PodJ L ) to a shorter form (PodJ S ) by a cell-cycle-regulated proteolytic sequence that eventually degrades PodJ S ensures proper localization and hence function of PodJ ( Viollier et al., 2002 ; Chen et al., 2006 ). ..

Synthesized:

Article Title: Cytokinesis signals truncation of the PodJ polarity factor by a cell cycle-regulated protease
Article Snippet: PodJ S is needed for chemotaxis and holdfast formation, presumably because, by analogy to PodJ L , it recruits polar factors required for those processes ( Viollier et al , 2002a ; Lawler et al , 2006 ). .. PodJ S is cleared from the cell pole during the swarmer-to-stalked transition, before PodJ L is synthesized again and localized to the opposite pole ( Viollier et al , 2002a ; Hinz et al , 2003 ; Chen et al , 2005 ). ..

Dissection:

Article Title: The Conserved Polarity Factor PodJ1 Impacts Multiple Cell Envelope-Associated Functions in Sinorhizobium meliloti
Article Snippet: .. Results from genetic dissection support this idea, as the periplasmic domain of PodJ L is required for pili production, while the cytoplasmic domain (of both PodJ L and PodJ S ) is involved in flagellar motility and holdfast formation ( Viollier et al. , 2002a ; Lawler et al. , 2006 ). .. One of the proteins recruited by PodJ to regulate organelle biogenesis is the PleC histidine kinase/phosphatase ( Viollier et al. , 2002a ; Hinz et al. , 2003 ; Lawler et al. , 2006 ), which is part of a signal transduction system that detects completion of cytokinesis and promotes distinct cell fates in the two progeny cells ( Matroule et al. , 2004 ; Paul et al. , 2008 ; Lin et al. , 2010 ).

Review